Science calculators

Enter the two plated dilution levels, replicate counts, TNTC plates, inoculum volume, and sample equivalent. The calculator estimates APC as CFU/mL or CFU/g and shows likelihood-based confidence limits.

Enter repeated OD600, CFU/mL, cell-count, or relative-biomass readings to fit one increasing modified Gompertz growth curve. The result estimates the fastest specific growth rate, lag time, plateau, doubling time, and when the fitted curve reaches a target.

Work backward from a target endpoint confluence to estimate the viable cells to seed today, then turn that dose into a per-well aliquot and a master-mix plan.

Enter a successful source run and the target rotor data to estimate the equivalent run time. You can use manufacturer k-factors or calculate k from rotor radii and operating speed.

Enter particle, liquid, and rotor geometry values to estimate whether the selected speed can pellet the target particles. The calculator also reverses the equation for required RPM and particle-size cutoff.

Enter the live and dead cells counted in full Improved Neubauer squares to calculate concentration, viability, and the volume to draw for a target cell preparation. The tool also checks whether the target can be made by dilution from the measured suspension.

Enter the wavelength, objective NA, magnification, adapter factor, camera pixel size, and recorded image dimensions. The calculator shows the optical resolution estimate, whether the camera samples it at Nyquist, the minimum magnification needed, and the captured specimen field.

Enter the stock concentration, target concentration, and final volume to find the stock volume and diluent volume for a ppm dilution.

Enter the mean Cq values from a serial dilution to fit a qPCR standard curve. The calculator reports the slope, amplification efficiency, R squared, residual error, and an estimated quantity for an unknown sample.

Enter the stock concentration, curve endpoints, replicate count, and pipetting limit to plan an equal-step qPCR standard dilution series. The result gives the dilution factor, tube volumes, retained template volume, and warnings for small transfers.